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Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy

文献类型:期刊文献

英文题名:Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy

作者:Li, Chun-yu[1,2];Wu, Xiao-yun[3];Tong, Jia-bei[4];Yang, Xin-xin[5];Zhao, Jing-li[6];Zheng, Quan-fu[1,2];Zhao, Guo-bin[7];Ma, Zhi-jie[8]

第一作者:Li, Chun-yu

机构:[1]302 Mil Hosp, China Mil Inst Chinese Med, Beijing 100039, Peoples R China;[2]Chengdu Univ Tradit Chinese Med, Sch Pharm, Chengdu 610000, Peoples R China;[3]Beijing Inst Life Sci, Translat Med Res Ctr, Beijing 100085, Peoples R China;[4]Shandong Acad Med Sci, Shandong Med Biotechnol Ctr, Jinan 250000, Peoples R China;[5]Changchun Univ Tradit Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China;[6]Jilin Vocat Coll Ind & Technol, Harbin 132013, Jilin, Peoples R China;[7]Hebei North Univ, Affiliated Hosp 1, Zhangjiakou 075000, Peoples R China;[8]Capital Med Univ, Beijing Friendship Hosp, Dept Pharm, Beijing 100050, Peoples R China

第一机构:302 Mil Hosp, China Mil Inst Chinese Med, Beijing 100039, Peoples R China

通讯机构:[1]Capital Med Univ, Beijing Friendship Hosp, Dept Pharm, Beijing 100050, Peoples R China.

年份:2015

卷号:6

外文期刊名:STEM CELL RESEARCH & THERAPY

收录:WOS:【SCI-EXPANDED(收录号:WOS:000355512000001)】;

基金:This work was supported by grants from the National Natural Science Foundation of China (grant no. 30850045).

语种:英文

摘要:Introduction: Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapies. Human platelet lysate represents an efficient alternative to fetal bovine serum for clinical-scale expansion of MSCs. Different media used in culture processes should maintain the biological characteristics of MSCs during multiple passages. However, bone marrow-derived MSCs and adipose tissue-derived MSCs have not yet been directly compared with each other under human platelet lysate conditions. This study aims to conduct a direct head-to-head comparison of the biological characteristics of the two types of MSCs under human platelet lysate-supplemented culture conditions for their ability to be used in regenerative medicine applications. Methods: The bone marrow-and adipose tissue-derived MSCs were cultured under human platelet lysate conditions and their biological characteristics evaluated for cell therapy (morphology, immunophenotype, colony-forming unit-fibroblast efficiency, proliferation capacity, potential for mesodermal differentiation, secreted proteins, and immunomodulatory effects). Results: Under human platelet lysate-supplemented culture conditions, bone marrow-and adipose tissue-derived MSCs exhibited similar fibroblast-like morphology and expression patterns of surface markers. Adipose tissue-derived MSCs had greater proliferative potential than bone marrow-derived MSCs, while no significantly difference in colony efficiency were observed between the two types of cells. However, bone marrow-derived MSCs possessed higher capacity toward osteogenic and chondrogenic differentiation compared with adipose tissue-derived MSCs, while similar adipogenic differentiation potential wase observed between the two types of cells. There were some differences between bone marrow-and adipose tissue-derived MSCs for several secreted proteins, such as cytokine (interferon-gamma), growth factors (basic fibroblast growth factor, hepatocyte growth factor, and insulin-like growth factor-1), and chemokine (stem cell-derived factor-1). Adipose tissue-derived MSCs had more potent immunomodulatory effects than bone marrow-derived MSCs. Conclusions: Adipose tissue-derived MSCs have biological advantages in the proliferative capacity, secreted proteins (basic fibroblast growth factor, interferon-gamma, and insulin-like growth factor-1), and immunomodulatory effects, but bone marrow-derived MSCs have advantages in osteogenic and chondrogenic differentiation potential and secreted proteins (stem cell-derived factor-1 and hepatocyte growth factor); these biological advantages should be considered systematically when choosing the MSC source for specific clinical application.

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