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Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods

文献类型:期刊文献

英文题名:Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods

作者:Kong, Wei-Jun[1,2];Zhao, Yan-Ling[1];Xiao, Xiao-He[1];Jin, Cheng[1];Li, Zu-Lun[2]

第一作者:Kong, Wei-Jun

机构:[1]China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China;[2]Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 610075, Sichuan, Peoples R China

第一机构:China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China

通讯机构:[1]China Mil Inst Chinese Mat Med, 302 Mil Hosp China,100 West,4th Ring Middle Rd, Beijing 100039, Peoples R China.

年份:2009

卷号:16

期号:10

起止页码:950-959

外文期刊名:PHYTOMEDICINE

收录:WOS:【SCI-EXPANDED(收录号:WOS:000270773600008)】;

基金:We are grateful to the support of National Basic Research Program of China (973 project) (2007CB512607 and 2006CB504703); Fond of State Youth Science (30625042) and National Natural Science Fond (No. 30772740). We thank the reviewers for their critical comments on the manuscript.

语种:英文

外文关键词:Rhizoma Coptidischinensis; UPLC-PAD; Quality control; Fingerprint; Chemometrics

摘要:To control the quality of Rhizoma Coptidis, a method based on ultra performance liquid chromatography with photodiode array detector (UPLC-PAD) was developed for quantitative analysis of five active alkaloids and chemical fingerprint analysis. In quantitative analysis, the five alkaloids showed good regression (R > 0.999 2) within test ranges and the recovery of the method was in the range of 98.4-100.8%. The limit of detections and quantifications for five alkaloids in PAD were less than 0.07 and 0.22 mu g/ml, respectively. In order to compare the UPLC fingerprints between Rhizoma Coptidis from different origins, the chemometrics procedures, including similarity analysis (SA), hierarchical clustering analysis (HCA), principal component analysis (PCA) were applied to classify the Rhizoma Coptidis samples according to their cultivated origins. Consistent results were obtained to show that Rhizoma Coptidis samples could be successfully grouped in accordance with the province of origin. Furthermore, five marker constituents were screened out to be the main chemical marker, which could be applied to accurate discrimination and quality control for Rhizoma Coptidis by quantitative analysis. This study revealed that UPLC-PAD method was simple, sensitive and reliable for quantitative and chemical fingerprint analysis, moreover, for the quality evaluation and control of Rhizoma Coptidis. (C) 2009 Elsevier GmbH. All rights reserved.

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